Assessment of phytochemical constituents, trace metals and antimicrobial efficacy of holy plant Couroupita guianensis, Southern India

Photochemical constituents, trace metals concentration and antimicrobial activity of (three different extract such as petroleum ether, chloroform and ethanol) C. guianensis leaves were investigated. The phytochemical screening of the crude extract revealed the presence of steroids, triterpenes, alkaloids, phenols, flavonoids, saponins and tannins. The leave powder was subjected to analyse the trace metals using 797 VA Computrace voltametry, Metrohm. The average mean concentrations of Cd, Cr, Cu, Fe, Ni, Pb and Zn were BDL, 0.02, 0.32, 0.89, BDL, BDL and 0.54 mg kg -1 , respectively. The three different solvents extract of C.guianensis were analyzed for in-vitro antimicrobial activity against certain pathogens and the zone of inhibition were compared with positive control such as Methicillin 10 mcg and Itraconazole 10 mcg.The Enterococcus fecalis and Trichophyton rubrum were most sensitive (19 mm) while smallest inhibition were recorded in Micrococcus luteus and Cryptococcus sp. (7 mm)

Problems in Public Distribution System

To minimize the poverty level among general public, Government of India promoted Public distribution system for extending basic necessary goods at subsidized rates. But their primary motto has not been fulfilled due to numerous problems prevailing in PDS. Thus, an attempt has been made in this study to ascertain the Problems prevailing in PDS. The result of the study indicates that Adulteration, Distribution of inferior quality goods, Under Weighment are the common problems found in Public distribution system

3D Modeling and Clash Deduction of PBS-Television Center, Hawaii

This experimental investigation carried out on the present mostly using software in construction Industries Revit which is software of Autodesk, Revit is building information modeling software for architects, structural engineers, MEP engineers, designers and contractors developed by Autodesk. It allows users to design a building and structure and its components in 3D, annotate the model with 2D drafting elements, and access building information from the building model#39s database. Revi is 4D BIM capable with tools to plan and track various stages in the building#39s lifecycle, from concept to construction and later demolition. This Project carried on the 3D modeling of a building which includes the design of Structural, Architectural, and Plumbing modeling. After modeling of the design this modeling was Plug Inn into the Navis Work Manage. It is also Autodesk software where we can find the Clashes and check the Quantity and Quality of the materials use in the construction. BY using this Softwarersquos in this project, we can give the detailed Information of the building structure and design before the construction in a detail. Where we can control the difficulties occurs during the working progress of the building, Wastage of the Materials can be deducted, The Clashes occurs at Walls, Beams, Footings, Columns, and we can see the Clashes between Structural and Architectural Plans and also between Structural and MEP (Mechanical, Electrical, Plumbing) plans and we can get the proper exact estimation of the Building materials and time of the project

समुद्री कूड़े में परिवर्तन और इस से तट रेखा में होनेवाला प्रभाव - कर्णाटक तट के संदर्भ में एक अध्ययन

कृपया पूरा लेखा पढ

Isolation, screening, and characterization of plant growth enhancing endophytic bacteria from halophytic Heliotropium curassavicum L. collected from salt stress areas of Srikakulam, Andhra Pradesh

Farmers use excessive chemical fertilizers to boost crop productivity to meet growing agricultural demands. However, this practice is costly and environmentally hazardous. Sustainable increase in crop yield can be achieved through alternatives like microbial-based fertilizers. In the quest to identify plant growth-promoting endophytic bacteria, the present study was carried out and selected unexplored halophytic plant Heliotropium curassavicum L. Thirteen endophytic bacterial strains were isolated from both aerial and root portions of H.curassavicum. These isolates were tested for salt tolerance, enzyme production, and synthesis of growth-promoting secondary metabolites, like Indole-3-acetic acid (IAA) and phosphate solubilization . Most of the isolates belonged to the Bacillus family, exhibiting varying Gram staining and biochemical reactions. The majority are Gram-positive bacteria, non-motile, spore formers, and exist in two cells or chains. All isolates could tolerate up to 10% NaCl concentration and a temperature of 42°C. Based on phenotypic, bio-chemical characteristics, isolate HCR3 showed promising properties in synthesizing IAA and phosphate solubilization abilities. The isolate HCR3 grew well upto 10% NaCl concentration and also 42°C temperature. Based on molecular characterization by using 16S rRNA gene-based analysis HCR3 isolate was identified and belonged to the Genus Pseudomonas with the highest similarity index with Pseudomonas khazarica sp. HCR3 showed IAA production of 37µg ml-1, had a phosphate solubilization ability of 3.5 ppm, and recorded protease activity on gelatin medium. The findings highlight the potential of HCR3 and other strains from halophytic H. curassavicum L. to enhance plant growth through secondary bioactive metabolites, offering eco-friendly solutions for sustainable agriculture

Screening of salt tolerant endophytic bacteria with plant growth promoting characters isolated from Acanthus ilicifolius L., a species of mangrove ecosystem located at Corangi wildlife sanctuary, Andhra Pradesh

Mangroves harbour many beneficial microorganisms in their rhizosphere, phyllosphere and endophytically, which forms an ideal ecological habitation for isolating halotolerant endophytic bacteria with unique characteristics. Endophytes can produce numerous bioactive secondary metabolites and phytohormones, which may be directly or in some way beneficial to the host plant.   The present study aimed to identify novel endophytes capable of producing plant growth-promoting substances. The mangrove plants Acanthus ilicifolius L. at Corangi Wildlife Sanctuary were selected, and their leaves and roots were collected for endophyte isolation. Eight isolates from the leaves and roots were collected, purified and preserved. All these isolates were subjected to morphological, phenotypical and biochemical studies. Isolates were grown best at 3% NaCl nutrient agar and could tolerate salinity upto 8%NaCl. Most of them could grow upto 42°C. The majority were gram’s positive, motile, aerobic, rod-shaped and some were gram’s negative, rod-shaped organisms. Many of the endophytic organisms had the ability to synthesize Indole-3-acetic acid(IAA) varied from 0.7 µg/ml (AIL1) to 51.0 µg/ml (AIL2) and the highest phosphate solubilizing ability was recorded with AIR3 (3.71 ppm) followed by AIR4 (3.00 ppm) and lowest was recorded by AIL4 (1.80 ppm). Among total isolates, AIL2 (51µg/ml) showed promising potential in producing IAA and had phosphate solubilization ability. Based on 16S ribosomal RNA molecular method the isolate AIL2 was identified as Bacillus altitudinis. This is the first to report that B.altitudinis strain AIL2 isolated from A.ilicifolius L. could produce IAA, which can be used as a bioinoculant in agriculture and allied sector

An interesting case of Phocomelia

Authors present a very rare case of tetra-phocomelia evaluated by antenatal ultrasonography. It is a condition seen in 0.62 per 100,000 live births. This is a congenital chromosomal abnormality involving the musculoskeletal system. Primi gravida with spontaneous conception after a long period of infertility underwent early anomaly scan. Patient was not aware of the last menstrual period hence; NT scan was missed. Routine early anomaly scan done between 16-18 weeks of pregnancy diagnosed a fetus with Tetra-Phocomelia. Due to the lack of associated symptoms or significant history, our case did not fit into any specific syndrome and appears to be the result of a sporadic, non-hereditary limb deficiency involving all four limb buds.  Second opinion obtained from a fetal medicine consultant who confirmed the diagnosis. Hence, decided for mid trimester termination and fetus was expelled after 8 hours. Fetus was not sent for pathological analysis. Tetra-phocomelia is a rare congenital anomaly and it may be associated with other deformity also. 1st case of phocomelia was described after the intake of thalidomide. In this condition hands and feet are seen as small flippers of a seal. The differential diagnosis includes sporadic phocomelia, Holt-Oram syndrome, thrombocytopenia-absent radius syndrome (TAR syndrome), Robert’s syndrome, and thalidomide-induced phocomelia. Here authors are presenting a rare case of Phocomelia where there is no history of drug intake or family history. This has to differentiate from thrombocytopenia-absent radius syndrome (TAR syndrome), sporadic phocomelia, Holt-Oram syndrome, Robert’s syndrome, and thalidomide-induced phocomelia